Volume 57 (2007) Issue: 2007 No#5-6

Characterization and antimicrobial activity of natural isolate Lactococcus lactis subsp. lactis BGSM1-19

Author(s): Strahinić Ivana, Cvetanović D, Kojić M, Fira Đ, Tolinački Maja, Topisirović Lj

Keywords:Lactococcus, bacteriocin, antimicrobial activity, Staphylococcus aureus, Micrococcus flavus

The strain Lactococcus lactis subsp. lactis BGSM1-19, isolated from traditionally homemade white cheese, produces two bacteriocins: lactococcin B-like bacteriocin named bacteriocin BacSMa and bacteriocin BacSMb which have shown similarity with lacticin RM. Plasmid curing resulted in a low yield (0.33%) of BacSMa– BacSMas and BacSMa- BacSMas, BacSMb-, BacSMbs derivatives. The bacteriocin biosynthesis was observed in the logarithmic phase of growth and the production plateau was reached after 8 h of incubation at 30oC, when the culture entered the early stationary phase. Biochemical characterization showed that strain BGSM1-19 retained antimicrobial activity within the pH range of 1 to 12 or after treatment at 100oC for 15 min. However, bacteriocin activity was completely lost after treatment with different proteolytic enzymes. The strain BGSM1-19 contains five plasmids. Plasmid curing indicated that genes coding for bacteriocins synthesis and immunity seem to be located on plasmids. BGSM1-19 exhibited antimicrobial activity against some pathogenic bacteria such as Salmonella paratyphi, Micrococcus flavus, Pseudomonas aeruginosa and Staphylococcus aureus.


My account

Search


Info

ISSN: 0567-8315

eISSN: 1820-7448

Journal Impact Factor 2017: 0.604

5-Year Impact Factor: 0.439

Indexing: Thomson Reuters/Science Citation Index Expanded, Zoological Record, Biosis Previews, Web of Science, Journal Citation Reports, Google Scholar, SCIndeks, KoBSON, Genamics, Journal Seek, Research Gate, DOAJ, Journal Rate, SJR – SCImago Journal & Country Rank, WorldCat, Academic Journals Database, Medical Journals Links, MedSci, Pubget

Contribute