Author(s): Tozon N, Nemec Svete A, Zemljič M, Zakošek M, Barlić-Maganja Darja
Keywords:FIV, FeLV, ELISA, PCR assays
The purpose of this study was to determine the prevalences of FIV and FeLV infections in domestic cats by the enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR). Of the 42 cats examined by the ELISA test, 14 (33.3 %) were seropositive for FIV antibody, 10 (23.8 %) for FeLV antigen, 1 (2.4 %) was positive for both viruses and 17 were negative for infection with either virus. In the PCR assay, 17 cats (40.5 %) were positive for FeLV, 11 (26.2%) for FIV, 9 (21.4%) for both viruses and 5 cats (11.9%) were negative for the presence of viral DNA. The average age of FIV positive cats was 6 and of FeLV 7 years. Infections were more common in outdoor cats (65% FIV, 61.1% FeLV positive) and in male cats (71.4% FIV, 80% FeLV). The results of both tests used in this study have shown high prevalence of both infections among cats in Slovenia. The ELISA method was found useful as a screening test for fast detection of FIV and FeLV infections, but additional testing with PCR was shown to be more effective in the characterization and progression of both infections.
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