Volume 58 (2008) Issue: 2008 No#2-3

Qualitative and quantitative parameters of swine cell immunity

Author(s): Popović Maja, Valpotić H, Kardum Paro Marijana, Pavičić Ž, Vijtiuk Nada, Popović Iva, Resanović Ružica, Valpotić I

Keywords:flow citometry, immunophenotypization, swine

Flow cytometry is a modern analytic method for qualitative and quantitive analyses of procaryotic and eukaryotic cells. Classical analytical methods examine one characteristic as an average for an examined cell population. Flow cytometry simultaneously measures several biochemical and physical parameters of every single cell in a heterogenic cell sample. Flow cytometry is routinely used all over the world in professional laboratories in veterinary medicine. For that purpose on the Veterinary Faculty of University in Zagreb, a machine for flow cytometry was standardized for use in veterinary medicine, especially for immunophenotyping of haemopoetic cells of domestic animals. In this manuscript we showed the standardisation method on a swine model, on samples of peripheral blood for the immunophenotypification of CD45+, CD3+ and CD21+ cells.