Volume 71 (2021) Issue: 2021 No#4

Genotypic correlation between Salmonella Enteritidis isolates from broiler breeders and hatchery flocks

Author(s): İnci Başak Müştak, Hamit Kaan Müştak, Seyyide Sarıçam İnce

Keywords:multilocus sequence typing, phylogeny, pulsed field gel electrophoresis, Salmonella

In this study, Salmonella Enteritidis strains isolated from dust and environmental materials from different flocks located in Turkey’s Western Black Sea region were examined by pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). A total of 59 S. Enteritidis strains isolated from broiler breeder and hatchery flocks, and one S. Enteritidis strain isolated from a stool sample of a farm worker were examined. PFGE analysis revealed two major PFGE groups and nine different macro restriction profiles. It was determined that 85% (51/60) of the strains were close to each other and comprised Group I. All S. Enteritidis strains had the same sequence type (ST): ST11. Isolation of strains with a single genotype suggests that there may be a cross transmission between the flocks.