Volume 53 (2003) Issue: 2003 No#2-3

COMPARISON OF METHODS TO DIFFERENTIATE STAPHYLOCOCCUS AND MICROCOCCUS SPECIES ISOLATED FROM BOVINE MAMMARY GLANDS

Author(s): Pengov A, Jurčevič A

Keywords:cow, laboratory methods, mastitis, Staphylococcus aureus

The hemolytic pattern of colonies, the slide coagulase (clumping factor) test, the tube coagulase test and the thermonuclease test were studied for their capability to differentiate Staphylococcus aureus from other Staphylococcus and Micrococcus species. A total 93 strains of Staphylococcus aureus isolated from bovine udder glands were tested. The hemolytic pattern of colonies and the slide coagulase test were found to be less reliable than other tests. No important differences could be found between the tube coagulase test and the thermonuclease test. The API-Staph test was used as a reference.