Author(s): Pavlović Sonja, Kovačević Grujičić Nataša, Mitrović Tatjana
Keywords:rat, -globin genes, transcription regulation, induction of erythropoiesis, REL cells
We have identified and characterized a DNA-binding activity with specificity for the -globin direct repeat elemnt (DRE) from rat b minyglobin promoter, an evolutionarily conserved transcriptional regulatory element in mammalian adult -globin promoters. We have also confirmed that the DRE contributed to the transcriptional inducibility of rat b miny- globin gene in rat erythroleukemia (REL) cells. By using gel mobility shift and South-Western blot competition studies we have shown that 60 kD erythroid-specific transcription factor and 35 kD and 20 kD ubiquitous factors from rat erythroleukemia cells specifically bind to the -DRE from the rat b miny- globin promoter. Additionally, a significant increase in the quantity of 60 kD transcription factor was observed upon erythroid differentiation of REL cells.
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