Volume 54 (2004) Issue: 2004 No#4

Cytogenetic changes in bone marrow and fibroblast cells of BALB/c laboratory mice induced by mestranol

Author(s): Teodorović Radislava

Keywords:cytogenetic changes, Mestranol, mouse, fibroblast, bone marrow cells

In this work the genotoxic effects provoked by Mestranol, which belongs to the group of chemical rodenticide- chemosterilants, were examined as a function of dose (7,5, 15 and 30 mg/kg w.b./c.c.) and of time (10, 20 and 30 days or 3, 5 and 7 days for "in vivo" and "in vitro" conditions, respectively). The genotoxic effects were scored on the basis of numerical and structural aberrations in both bone marrow and fibroblast cells from BALB/C laboratory mice in comparison to control groups. Increased doses of the preparation increased numerical aberrations in both bone marrow and fibroblast cells significantly (p<0.01) so that the greatest changes were noticed after the treatment with 30 mg/kg w.b./c.c. Numerical aberrations showed no significant differences (p<0.05) as a function of time. Also, the several times greater number of aneuploid cellss was significantly different (p<0.01) compared to the number of polyploid cells Greater values for structural aberrations were obtained not only after the treatment with the largest dose of 30 mg/kg w.b./c.c., but also after longer exposure times (i.e. for 30 or 5 days respectively). Statistically significant differences were found between the number of gaps, as well as fragments, in comparasion to the number of Robertsonian translocations (p<0.01), while there were no significant differences between thenumber of gaps and fragments (p>0.05).