Author(s): Jovanović S, Trailović Ružica, Savić Mila, Sarač M
Keywords:wild pigs, PSS, halothane test, RYR1 locus
Expression of the stress syndrome (PSS) in European wild pig was evaluated by Halothane test and tested animals were typed by PCR/RFLP test for C/T mutation at nt 1843 in RYR1 gene. A total of 56 animals, at the age of 5 months, weighing 20 kg average, were tested. Hal+ (MHS – malignant hyperthermia susceptible) phenotype was revealed in 6 pigs, 47 animals expressed Hal– (MHN – malignant hyperthermia resistant) phenotype, while 3 animals showed no response to administration of 3% halothane after 5 minutes and they were marked as Halr (halothane resistant) phenotypes. Genotyping of 56 wild pigs revealed that the tested population was homozygous for the normal C/C allele at RYR1 locus. The results obtained by halothane test show that expression of the PSS in wild pigs is modulated and influenced by factors other then RYR1 genotype.
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